A comparative time course analysis of green fluorescent protein reporter-gene expression and DNA uptake was conducted to quantitatively compare PEI- and Au PAMAM-mediated transfection in CT26 and SK-BR3, while flow cytometry and confocal microscopy were used to determine the contribution of cellular uptake, endosomal escape, and cytoplasmic transport to the overall gene delivery process.Results from the time course analysis and flow cytometry studies revealed that initial complex uptake and cytoplasmic trafficking to the nucleus are likely the two main factors limiting CT26 transfectability.Medical physics, synthesis and characterization of nanoparticles, development of nanoparticle based systems for multimodal imaging and therapeutics, nanoparticle based radiosensitizers, drug delivery, intracellular fate of nanoparticles. v=ISZMq KOvn No Nanotechnology involves creation and utilization of materials, devices or systems on the nanometer scale. The field of nanotechnology is currently undergoing explosive development on many fronts. Unresolved questions include the detailed molecular uptake mechanism(s), reasons for cell toxicity, and the delivery efficiency of CPPs for different cargoes.Here, we give a review focused on uptake mechanisms used by CPPs for membrane translocation and certain experimental factors that affect the mechanism(s).However, these drug delivery nanosystems have shown some limitations regarding the toxicity of the nanoscale materials in the body.In order to reduce their toxicity, it is crucial to study endocytosis, exocytosis, and clearance mechanisms for nanoparticles released from the nanoparticle–drug conjugates.
Recently, much attention has been given to the problem of drug delivery through the cell-membrane in order to treat and manage several diseases.
The cell membrane is the structure that protects living cells from the surrounding environment, only allowing the movement of compounds generally with small molecular size across this barrier into the cell.
Some drugs are large hydrophilic molecules showing major limitations for their penetration through the cell membrane.
My research program is designed to improve the understanding of the bio-nano interface.
Better knowledge of the nano-bio interface would lead to better tools for diagnostic imaging and therapy.